A publication of the Foresight Institute
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Scanning tunneling microscopes have been used to to modify
individual molecules. Their main advantage is that they allow
modifications at precisely chosen locations. Their main
disadvantage is that they operate on only one molecule at a time.
The first paper mentioned below describes a technique which may
extend the range of structures that can be built with an STM,
while the second demonstrates the stability of a type of switch
that can now be built with an STM.
An advance in controlling the environment of a scanning tip, which may be applicable to fabrication, came from J. D. Noll, P. G. Van Patten, M. A. Nicholson, K. Booksh, and M. L. Myrick, writing in [Rev. Sc. Instrum. 66: 4150-4156 Aug95]. They built a fluid cell for a scanning tunneling microscope that allowed them to exchange fluids during imaging. The noise due to fluid flow is sufficiently low that they were able to image graphite with atomic resolution during flow. From a fabrication viewpoint, their cell should allow a sequence of liquid phase reactions and scanning probe controlled workpiece modifications to be applied to a single site. One should, for instance, be able to adsorb one reagent on a surface, then locally modify it with the scanning tip, then adsorb a different reagent, then modify the new layer of the second reagent at the same site, and so on. The stability of the fluid cell that this group has demonstrated should allow such a sequence to be followed without needing to relocate the work site after changing reagents, as would occur with a more disruptive method for changing reagents. In the cell described in this paper, a reagent injected into the fluid stream reached the cell approximately 90 seconds later, and peaked in concentration about 50 seconds after that, setting a time scale of several minutes for replacing reagents in this design.
An STM-based approach to nanometer-scale circuits is described by D. P. E. Smith in [Science 269: 371-373 21Jul95]. This paper describes experiments in switching quantum conduction channels on and off in an STM experiment. The experiments were performed at 4.6 - 8.6 K, with contact between a gold ball and a nickel tip (thought to become coated with gold, producing gold-gold contacts, under experimental conditions). The key finding is that the contact can form a very stable high conductance state, with a conductance of 0.977▒0.015 (2e2/h). This conductance "could be stably measured over one position for a period greater than 24 hours and is in good agreement with detailed simulations that find a stable single-atom contact with a quantized conductance of 0.93▒0.05 (2e2/h)." The junction could be repeatedly cycled between the conducting state and a state with about 15 times as much resistance. The author observed switching speeds as fast as 10Ásec (limited by sensing electronics), and noted that "Molecular dynamics simulations have shown that the fastest possible switching time for single atoms in the point-contact configuration is on the order of 1 psec." The author also observed switching of a second conductance channel, but much less stably than the switching of the first channel. Because a long conductance channel would make the switching of the second channel more distinct, "...the single-channel constriction probably corresponds to a single atom." Presumably the reproducible cycling between states of a single channel shows that one of the bonds to an atom at the channel constriction can be repeatedly broken and reformed without damaging nearby bonds enough to change the resistance. In addition "The QPC [quantum point contact] switch displays power gain and can be used to make an oscillator (by feeding the output signal back into the control input) or to drive a series of other QPC switches."
Logic devices are necessary components of intelligent
manufacturing systems. The paper described below shows one
approach to using small numbers of electrons to operate
electronic circuitry, including logic devices.
W. R÷sner, F. Hofmann, T. Vogelsang, and L. Risch write of a technique for simulating certain classes of single electron circuits in [Microelec. Eng. 27: 55-58 1995]. They model their circuits as a set of electrical nodes, tied together by capacitors and tunnel junctions, with a charge on each node due to a small number of electrons.
Their modeling process calculates the electrostatic voltages on each of the nodes. For each tunneling junction, it then calculates the change in energy that would be produced by a single tunneling event through the junction. Based on this energy change and the resistance of the junction, they then calculate the rate at which electrons tunnel through it. The actual transition is then selected with a probability proportional to its rate. Because this analysis is applied to circuits with very small capacitances, the unfavorable changes in energy due to the movement of individual electrons are accounted for, so "Coulomb blockade" effects are properly modeled. Using their program, the authors have modeled the voltage transfer curve of a logic inverter, and have also simulated a ring oscillator built with 3 stages of inverters. This simulated oscillator operated at a frequency of 20GHz with a power consumption of 3nW. While this dissipation is larger than that possible with fully atomically precise fabrication, it looks like an attractive intermediate target.
The authors describe some limitations to this modeling technique. It neglects the energy level structure within the conducting regions that form the nodes of the circuits. They note that this is acceptable as long as the regions are a few nm in size, which makes the energy level separations smaller than the voltages used in the circuits (67 mV was used in the inverter simulation). In terms of fabrication, this makes the circuits designed with this paradigm relatively insensitive to the atomic scale details of their conducting regions. The calculation of the tunneling rates is also approximate, depending only on the characteristics of a single junction at a time. This is accurate as long as the junction resistance is large compared to the quantized resistance h/e2 = 25.8kohms. In terms of fabrication, this makes the circuit independent of quantum phase relationships across several circuit elements. These circuits do need atomically precise control of their tunnelling junctions, since the detailed bond geometry in the junction sets the tunnelling resistance. The circuits might be good targets for a hybrid technique where the tunnel junctions are built with atomic precision by classical chemistry, and are then attached to capacitors and interconnections built by STM lithography.
The self-assembled route to nanotechnology requires extending
synthetic structures to larger sizes in order to form working
mechanisms. This, in turn, requires highly specific synthetic
steps to maintain tolerable yields of large, complex structures.
These requirements are generally better satisfied by enzymatic
(or catalytic antibody) reactions than by classical chemistry.
Applications in molecular manufacturing have additional
requirements. They also require stiff structures, partially to
reduce the effects of thermal noise on mechanisms and (in the
more extreme case of polymers with freely turning bonds) to keep
the task of predicting folding possible. In addition, it is
important that there be enough flexibility in the synthesis
techniques to permit the design of a useful range of structures.
The papers described below extend biosynthetic capabilities in
A new DNA technology that extends the range of metabolic products available is described by M. Rouhi in [C&EN p9 2Oct95]. ChromaXome cofounder K. A. Thompson explains "...if you take DNA and paste it to DNA from another source or from other places in the same bacteria, then you get a chemical that's built from a combination of pathways..." From the point of view of nanotechnologists, these might provide building blocks which are not otherwise available. P. B. Fernandes (a vice president at Bristol-Myers Squibb) says "The diversity from natural products is much more than you can buy from synthetic chemistry."
A specific example of which pathways might be usefully combined is explored by R. McDaniel, S. Ebert-Khosla, D. A. Hopwood, and C. Khosla writing in [Nature 375: 549-554 15Jun95]. They describe the biosynthesis of polyketides, polymers of -(C=O)-CH2-, and their derivatives. The polyketide backbone, without modification, would have many bonds about which it could twist, and would not be a particularly good candidate for building stiff structures. Fortunately, polyketide derivatives include compounds where the backbone has been cyclized into sets of fused aromatic rings. One of the new products that the article focuses on (designated SEK26) is a substituted anthraquinone (a three ring structure). The authors give a set of design rules for which gene clusters can be combined to give a functional synthetic pathway. At present, 8 backbones are available, with the prospect that "enzymes that catalyze downstream cyclizations and late-step modifications, such as group transfer reactions and oxidreductions commonly seen in naturally occurring polyketides, can be studied along the lines presented here and elsewhere." Hopefully, some of the products of these enzymatic pathways may include stiff, fused ring structures, valuable for constructing atomically precise mechanisms, that are not available from classical organic chemistry. Alternatively, the active sites of the enzymes which synthesize stiff, fused ring structures may serve as a model for extending an early, protein-based nanotechnology towards the diamondoid structures that exhibit better mechanical properties and wider design options.
Turning to a broader range of biochemically catalyzed reactions, P. G. Schultz and R. A. Lerner, writing in [Science 269: 1835-1842 29Sep95] describe the state of the art in catalytic antibodies. Catalytic antibodies are raised by provoking an immune response to a compound that resembles the transition state of a reaction that one wants to accelerate. In catalysis, the antibody binds to the transition state of the reaction, stabilizing it, lowering the activation energy of the reaction, and accelerating the reaction. One of the early reactions to be catalyzed was ester hydrolysis. The transition state for hydroxyl attack on an ester has a tetrahedral carbon at the carboxyl position. Analogous phosphonates have sufficiently similar tetrahedral geometries and charge distributions that antibodies to them can catalyze hydrolysis of esters.
Shultz and Lerner describe a variety of reactions which are now possible with catalytic antibodies. One of their examples is the catalysis of a Diels-Alder addition ("...in its simplest form...the reaction of butadiene and ethylene to yield cyclohexene..."). This is particularly notable because no natural enzymes are known which catalyze this reaction. More generally, Shultz and Lerner describe catalysis of a class of reactions known as pericyclic reactions, only one of which is known to have an enzyme that catalyzes it. "These reactions have not only received a great deal of theoretical and mechanistic attention from chemists, they have also found many applications in organic synthesis." In addition to the Diels-Alder reaction, they describe catalysis of the Cope rearrangement, an intramolecular rearrangement which moves two double bonds and a single bond in a six-atom group.
Another class of reactions that Shultz and Lerner cover is the catalysis of reactions involving reagents ("cofactors") that are not present under physiological conditions. The examples that they give are catalysis of the oxidation of an organic sulfide by periodate and the reduction of a number of ketones by cyanoborohydride to the corresponding alcohols with "96% enantiomeric excess." In the corresponding uncatalyzed reaction, there would be, of course, no selectivity between enantiomers.
Some reactions that can now be catalyzed have sufficiently unstable transition states that they do not occur at any appreciable rate in the absence of catalysis (although undesired side reactions of the same reagents may occur without catalysis). An example of a cyclization is given where the product formed in the presence of the catalytic antibody is essentially absent in the normal reaction products. Another example is given where a cyclization product is formed in 98% yield where the uncatalyzed reaction yields an impure mixture of many products. The authors write "These studies will undoubtedly lead to efforts aimed at larger multiring cyclization reactions."
The facility that these antibodies provide to nanotechnologists is the ability to produce more selective reactions than classical organic chemistry allows. Since the antibodies can bind all over the surfaces of their substrates, they can geometrically orient the reactants, confining them to just one of a number of potentially competing reaction pathways. This can potentially allow us to build structures which are unreachable (in reasonable yields) through classical techniques.
Another approach to controlling stiff, polycyclic structures from a technology base which initially controls only flexible compounds is to control the formation of the polycyclic structures in crystal lattices. A. Berman, D. J. Ahn, A. Lio, M. Salmeron, A. Reichert, and D. Charych, writing in [Science 269: 515-518 28Jul95], describe controlling the crystallization of calcite with an organic monolayer. Their experiments grew calcite crystals on an acidic PDA (polydiacetylene) film. The monomer that they used was CH3-(CH2)11-CC-CC-(CH2)8-COOH. Polymerization of this compound yields long polymer chains within the PDA monolayer. In these experiments the calcite crystal's a axis was aligned "parallel to the polymer backbone direction" (within the plane of the film). By comparison, in previous examples of crystal nucleation on thin films "The crystal axes in the plane of nucleation do not appear to be aligned with a structural parameter of the nucleation surface." The authors state that "the total control exerted over their [the crystals'] orientation is to our knowledge unprecedented in crystal growth at the in vitro organic-inorganic interface." In order to use these crystals for atomically precise mechanisms, it would now be desirable to extend this work to control the absolute position, as well as the orientation, of these crystals, perhaps by performing the analogous experiments on proteins that mimic a finite, well-defined area of the PDA membrane.
An important capability in approaching nanotechnology is to be
able to evaluate the results of attempts to build target
structures. A three dimensional reconstruction of the structure
of the ribosome at 25┼ resolution is described by J. Frank, J.
Zhu, P. Penczek, Y. Li, S. Srivastava, A. Vershoor, M.
Radermacher, R. Grassucci, R. K. Lata, and R. K. Agrawal in [Nature
376: 441-444 3Aug95]. The analysis technique that they
used combined 4,300 electron micrographs of separate ribosomes.
The computational power brought to bear on the problem was
remarkable. For instance, "The orientation of each
projection was determined individually by matching it to computed
projections of the previous 29┼ model..." This analytical
technique may prove useful for determining if some self-assembly
step succeeded or failed. It retains one of the strongest points
of x-ray diffraction, the ability to use information from many
molecules, without requiring the ability to precisely align all
of the molecules in a crystal, as x-ray diffraction requires.
The specific results of this group allow them to identify "a channel in the small ribosomal subunit and a bifurcating tunnel in the large subunit which may constitute pathways for the incoming message and the nacent polypeptide chain, respectively." As this model is extended towards molecular precision, it may allow us to improve the yield of our in vitro peptide synthesis techniques, or possibly to extend the capabilities of ribosomes in intact organisms. Either of these developments would broaden the range of proteins available to us for constructing novel structures.
Jeffrey Soreff is a researcher at IBM with an interest in nanotechnology.
It was a pleasure addressing those of you who attended the Foresight
Conference on Molecular Nanotechnology in mid-November. Your
questions, as usual, were thought- provoking and perceptive. I
enjoyed the dialog. Unfortunately, there was more to discuss than
time available for my presentation. This column will fill in the
Primarily, you should be aware of a few pending bills that may have additional impact on emerging technology, such as nanotechnology. The House Bill HR 1733, sponsored by Rep. Carlos J. Moorhead (R-CA) and Patricia Schroeder (D-CO), proposes to amend the patent term from its present 20 years from the U.S. priority filing date, to 20 years from the filing date of the issued patent. This is a subtle, yet significant difference; 20 from priority means that the 20 year term may be measured from an earlier patent application from which the issued patent is derived; whereas 20 years from the filing date of the issued patent merely considers the date on which the issued patent was filed, independent of when the parent case was filed. To overcome issues of "submarine patents", this bill includes a mandatory publication of patent applications at 18 months from the priority filing date.
For example, an applicant files a "parent" application on June 30, 1995, and subsequently files on July 30, 1996, a "continuation" or a "divisional" application based on the parent case. Under the existing law (as amended post-GATT) the issued patent would have a term of 20 years from June 30, 1995, but under the Moorhead/Schroeder Bill, the patent would have a term of 20 years from July 30, 1996, with a publication occurring December 30, 1996.
Another House Bill HR 359, sponsored by Rep. Dana Rohrabacher (R-CA) proposes to change the patent term to the longer of 17 years from issuance or 20 years from the U.S. priority filing date. However, because it does not address concerns over "submarine patents", it is not expected to succeed.
Another controversial pending House Bill is HR 2235, also sponsored by Moorhead. That Bill proposes to grant certain rights to prior users of patented technology. Such a provision exists under most foreign patent systems, and is notably absent from the U.S. system. The Bill proposes to grant a royalty-free right to a prior user to continue using the patented invention if the prior user has been using it in good faith, and only continues to use technology for the use existing at the time the patent issues. Obviously, this Bill raises both significant concerns and potential for small companies, individual inventors, and emerging technologies.
This last topic is worth discussing, and I welcome your comments. I will address this issue, incorporating your comments, in the next column.
Elizabeth Enayati is a patent law specialist with the Palo Alto law firm Weil, Gotshal & Manges.
Her e-mail address is email@example.com, or phone (415) 926-6248, or fax (415) 854-3713. She can also be reached by mail c/o Foresight Institute, P.O. Box 61058, Palo Alto, CA 94306.
[Editor's Note: See later columns for Elizabeth Enayati's new affiliation.]
"There is something very wrong with American
science." This provocative claim was offered by George A.
Kenworth II, White House Science Advisor under the Reagan
Administration, while addressing the House Science Committee last
June. "Preserving the status quo has become the overarching
goal, replacing the pursuit of excellence," he continues. In
addition, U.S. science suffers from "a deeply ingrained lack
of [public] accountability" and "ingratitude for two
generations of unparalleled federal largesse.
"A major overhaul is needed." And the restructuring implicit in the creation of a Department of Science (DOS) "is the only way I know to restore coherent policies, research dedicated to excellence, and the public trust," Kenworth told the committee.
Robert S. Walker (R-Pa.), chair of the committee, anticipates drafting a bill to establish a DOS to the U.S. Congress. He estimates that the coordination and streamlining of policy made possible by merging many research responsibilities could, over seven years, save Uncle Sam more than $2 billion and eliminate more than 5,000 federal jobs.
While the proposal won overwhelming endorsement from most committee members present - and from all invited witnesses - the June 28th hearing also highlighted some major issues that must be resolved before the long-term implications of such a restructuring can be evaluated. Turf battles are expected.
One issue sure to kindle protectionist passions among people currently engaged in federal research and development (R&D) is how much applied research - the "D" in R&D - a DOS should undertake. Today, nearly 80% of the federal R&D budget goes to applied research, including technology development.
Some people anticipate a DOS would probably spell an end to the White House Office of Technology Policy (OSTP), but one of the chief advantages of a DOS would be to merge science policy making and program implementation into a single structure. [Science News, 148: 59-60]
In the U.K., an ambitious program to harness science,
engineering and technology for increasing the competitiveness of
that nation's businesses is now ready to disseminate and
implement a number of recommendations. These recommendations are
based on a massive consultation exercise spanning 15 sectors of
British industry. One of the more concrete proposals is for the
creation of a national institute for applied catalysis.
The program is known as "Technology Foresight," and was one of several announced in a 1993 document titled "Realizing Our Potential: A Strategy for Science, Engineering, and Technology." It was the first major policy review in the area for 20 years. "The program is a new departure," says John Brophy, research general manager for BP Chemicals, Sunbury on Thames, England. "It identifies national priorities for the scientific infrastructure to channel research in the direction of industry." Brophy is vice chairman of the Technology Foresight Panel on Chemicals. He explains "Our panel recommends that we retain a large element of that, but also ensure that the science base, working with industry, is prepared for and aware of market opportunities when they arise."
Top priority areas include genetic and biomolecular engineering, sensors and sensory information processing, and environmentally sustainable technology. Catalysis, chemical and biological synthesis, and materials are regarded as intermediate priority areas that require further action and development of "stronger exploitation links." The strategic themes include the need for a cleaner, more sustainable world, and advances in materials science, engineering, and technology, with a particular emphasis on multidisciplinary settings. [C&EN July 3, 1995, pps. 16-17]
"A Visualization Revolution" is how recent advances
in computing, visualization algorithms, and display technology
are heralded by R&D Magazine, in the October
1995 issue. "Virtual environments" can now be created
which enable researchers to make prototypes and conduct tests and
experiments. A virtual prototyping system, with stereoscopic
computer-driven images projected on two walls and the floor of
the CAVE (Cave automatic virtual environment) has been unveiled
at the National Center for Supercomputing Applications at the
University of Illinois, Urbana-Champaign.
Researchers at Caterpillar Inc., have developed a virtual reality copy of Caterpillar's Peoria test track. In the CAVE, one can sit in an instrumented mockup of an earth-moving machine cab. Listening to lifelike stereo sounds, the driver maneuvers the computer-driven machine and its earthmoving accessories. With these simulations, Caterpillar engineers can eliminate the lengthy process of fabricating steel casting needed for design studies by changing CAVE's design program, which only takes a few hours.
Recent advances in desktop computing power and visualization of algorithms mean that supercomputers are no longer always necessary. An engineer with Pacific Gas & Electric used a desktop system and graphical analysis program to study the effects of earthquakes on a building. Using accelerograms to study the effects of the 7.1 Richter scale Loma Prieta earthquake on a 34-story steel building in San Francisco, the model resulted in visualizations of the movement of the 34th floor during Loma Prieta. These data allow structural engineers to realistically evaluate the seismic safety of the building. The analyses included baseline corrections, integration to obtain velocity and displacement data, cross-section functions, and fast Fourier transforms to measure the response to earthquake movements.
In line with my unbridled enthusiasm for the Foresight Web Project, here are two important URLs for Update readers.
Color microscopy imaging is now seen on Web pages to add product details and provide users with application forums. Many of these images would interest nanophiles. Point your Web browser to:
Digital Instruments, http://www.di.com
Dr. Jamie Dinkelacker leads Apple Computer's development of multimedia authoring tools for science, math, and medical education as Senior Engineer Scientist, Technical Manager of the East/West Authoring Tools Group within Apple's Advanced Technology Group.
Newly devised computational algorithms make possible more
complex and useful simulations of nanoscale structures, Dr.
Donald Noid told the second meeting of the Minnesota Molecular
Nanotechnology (MNT) Study Group. Dr. Noid is a physical chemist
from the Department of Energy's Oak Ridge National Laboratory and
pioneer of chaos theory.
Recent molecular modeling work by the Oak Ridge National Laboratory trio of Donald W. Noid, Bobby G. Sumpter and Robert E. Tuzan also was described in a recent presentation by Drs. Sumpter and Noid at Technology 2005, held at Chicago's McCormick Place convention center. Sumpter and Noid presented the trio's paper, "Advancing Manufacturing Through Computational Chemistry," which discusses the path toward convergence of nanotechnology and computational chemistry.
In the Minnesota presentation, Dr. Noid described his molecular simulation software and showed a video of the software simulating several new molecular-scale mechanisms. His algorithm reduced the computation required from a page full of equations per atom simulated to just a few lines, he said. This algorithm makes computational simulation of a many-atom structure possible. Without it, many of the simulations he has done would be impractical with currently available computing power.
Noid's videos provide a sense of watching actual atoms. We can
"see" atoms, make things out of them, and see if they
work. He said his software makes simulations more realistic by
taking into account conservation of energy. All other known
molecular dynamics packages have systematic errors under which
energy monotonically increases with time.
Dr. Noid believes that simulations of a mechanism must run for a fair amount of time, several nanoseconds at least. He has seen a structure act in a very stable fashion for a long period, then exhibit anomalous behavior. With his software, you can "fine tune" the molecular structure with successive iterations to reduce or eliminate unwanted behavior, in the process developing "design rules" which, if followed, will result in sound mechanisms. With this software, it is as if we could suddenly make any physically possible molecular structure, and let it run and watch it work or not. This is enormously powerful.
He cited two problems doing large simulations (thousands of atoms for millions of simulation cycles). First is the substantial computing power required. His algorithms provide much more accurate results (especially for long simulation times), and do it much more efficiently, but the shear size of the computations required is still significant. Some of his simulations required as much as a week on up to four IBM RISC 6000 processors (a mini-supercomputer costing around $60,000 each). Fortunately, his code is extremely vectorizable, meaning that it can be run on many processors in parallel very efficiently. This allows efficient simulation on even the most massively parallel machines, such as the Paragon.
Another problem with large simulations is the large amount of data generated. This problem is not unique to molecular simulations. People have been trying to deal with simulation results data since the first simulators were used, several decades ago. He is currently working on ways to "mine" that data for useful information, using neural nets and other approaches.
Steven C. Vetter (firstname.lastname@example.org) is a co-founder and President of Molecular Manufacturing Enterprises, Inc., 9653 Wellington Lane, Saint Paul, MN 55125. MMEI is working with Dr. Noid to make his software more widely available.
From Foresight Update 23, originally published 30 November 1995.