Nanotechnology may bring the $100-dollar personal genome in five years

By combining a nanofluidics device to stretch out a single long DNA molecule with a strategy to read five DNA letters at a time, two companies are applying nanotechnology to develop a really cheap method to sequence individual genomes to make possible individualized medicine. From “The $100 Genome“at Technology Review, written by Emily Singer, via

Forget the $1,000 genome. Some companies are looking far past that goal to create a really inexpensive sequencing technology.

It currently costs roughly $60,000 to sequence a human genome, and a handful of research groups are hoping to achieve a $1,000 genome within the next three years. But two companies, Complete Genomics and BioNanomatrix, are collaborating to create a novel approach that would sequence your genome for less than the price of a nice pair of jeans–and the technology could read the complete genome in a single workday. “It would have been absolutely impossible to think about this project 10 years ago,” says Radoje Drmanac, chief scientific officer at Complete Genomics, which is based in Mountain View, CA.

The most recent figures for sequencing a human genome are $60,000 in about six weeks, as reported by Applied Biosystems last month. (That’s down from $3 billion for the Human Genome Project, which was sequenced using traditional methods and finished in 2003, and about $1 million for James Watson’s genome, sequenced using a newer, high-throughput approach and released last year.) But scientists are still racing to develop methods that are fast and cheap enough to allow everyone to get their genomes sequenced, thus truly ushering in the era of personalized medicine.

Most existing technologies detect the sequence of DNA a single letter at a time. But Complete Genomics aims to speed the process by detecting entire “words,” each composed of five DNA letters. Drmanac likens the technology to Google searches, which query a database of text with keywords. Further speeding up the process with novel chemistry and advances in nanofabrication, the companies will develop a device that can simultaneously read the sequence of multiple genomes on a single chip.

…Each DNA molecule will be threaded into a nanofluidics device, made by Philadelphia-based BioNanomatrix, lined with rows of tiny channels. The narrow width of the channels—about 100 nanometers—forces the normally tangled DNA to unwind, lining up like a train in a long tunnel and giving researchers a clear view of the molecule. “Since we can stretch out DNA, we can get a huge amount of information from each piece of DNA we look at,” says Mike Boyce-Jacino, chief executive officer of BioNanomatrix. “The big difference from any other approach is that we are looking at physical location at the same time we are looking at sequence information.” Sequencing methods currently in use sequence small fragments of DNA and then piece together the location of each fragment computationally, which is more time consuming and requires repetitive sequencing.

The companies still have a long road to the $100 genome. BioNanomatrix has already shown that long pieces of DNA—two million letters in length—can be threaded into the channels of existing chips. But now researchers need to develop chips with many more channels, so that multiple genomes’ worth of DNA can be sequenced simultaneously.

…The technology necessary to achieve a $100 genome is still at least five years away, says George Church, a geneticist at Harvard Medical School, in Boston, and a member of Complete Genomics’ scientific advisory board. “But [it’s] coming from a company that has an almost-as-good technology coming out this year.”

I am happily surprised to see something as conceptually simple as using fluid flow to uncoil and align a large molecule solve a problem as seemingly difficult as how to work on individual DNA molecules. Perhaps nanofluidics will also be useful for other macromolecular assembly tasks along the road to productive nanosystems.

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